Journal: Molecular Biology Reports

Article Title: Regenerated silica-based RNA purification columns to address the short supply of RNA purification kits for COVID-19 diagnosis

doi: 10.1007/s11033-021-06688-0

Figure Lengend Snippet: Regenerated Viral-RNA purification kit columns have a comparable capacity for SARS-CoV2 RNA purification as the fresh columns. Bars represent the mean ± standard deviation Ct values for each SARS-CoV2 RT-qPCR target genes RdRp, E, N, and IC, for swab samples with Ct value ≤ 25 ( A ), Ct value = 25–35 ( B ), and Ct value = 36–40 ( C ). Each dot represents one sample. Pairwise comparisons of mean Ct values for each SARS-CoV2 target gene were done using a two-tailed paired Student’s t-test, with a confidence level of 95%. For low Ct value samples p value for RdRp, E and N genes were 0.18, 0.59 and 0.75 respectively, for moderate Ct value samples p value for RdRp, E and N genes were 0.57, 0.65 and 0.47 respectively, and for high Ct value samples p value for RdRp, E and N genes were 0.84, 0.85 and 0.86 respectively

Article Snippet: In our study, the method of regeneration and re-use of used RNA-purification columns for COVID-19 diagnosis was also extended for RNA-binding silica columns from two commercial sources (HiPurATM Viral RNA Purification Kit, HIMEDIA, and PureLink RNA Mini Kit, Invitrogen, Thermo).

Techniques: Purification, Standard Deviation, Quantitative RT-PCR, Two Tailed Test

Journal: NPJ Vaccines

Article Title: Selection and evaluation of an efficient method for the recovery of viral nucleic acids from complex biologicals

doi: 10.1038/s41541-018-0067-3

Figure Lengend Snippet: Optimization of sample-processing pipeline. The two major areas of focus for sample-processing optimization were nucleic acid extraction and double-stranded DNA synthesis. Total nucleic acid extraction and separate extraction of DNA and RNA were explored. The extracted nucleic acid was converted to single-stranded DNA before double-stranded DNA synthesis using whole-genome amplification or second-strand synthesis

Article Snippet: Where the double extraction method was used (Invitrogen PureLink™ Viral RNA/DNA kit and the Wako DNA Extractor® kit with RNase A digest), the initial sample was divided into two equal portions and used for extraction using each of the kits.

Techniques: DNA Synthesis, Whole Genome Amplification

Journal: NPJ Vaccines

Article Title: Selection and evaluation of an efficient method for the recovery of viral nucleic acids from complex biologicals

doi: 10.1038/s41541-018-0067-3

Figure Lengend Snippet: Efficiency of separate extraction of DNA and RNA when compared to total nucleic acid extraction using QIAamp® MinElute® Virus Spin kit. DNA and RNA were extracted separately and compared to the total nucleic acid extracted using the QIAamp® MinElute® Virus Spin kit, using qPCR. RNA extraction using the Qiagen RNeasy® Mini kit did not enhance the detection of the two single-stranded RNA viruses (FeLV and RSV), whereas the RNase A digestion added to the Wako DNA Extractor® kit greatly enriched for double-stranded nucleic acid (EBV and Reo3)

Article Snippet: Where the double extraction method was used (Invitrogen PureLink™ Viral RNA/DNA kit and the Wako DNA Extractor® kit with RNase A digest), the initial sample was divided into two equal portions and used for extraction using each of the kits.

Techniques: RNA Extraction